Hvar is the longest island in the Adriatic Sea. It belongs to the group of central Dalmatian islands, and by area it is the fourth largest island in the Adriatic. Administratively, it is divided into four parts: the town of Hvar, the town of Stari Grad, the municipality of Jelsa and the municipality of Sućuraj.

Hvar is only one of the destinations of field research of the Institute of Anthropology, and it contains data on the population structure (differentia specifica of the Institute) collected over 40 years on island isolates.

In the year 1979. we collected 1526 questionnaire / respondents from Vrisnik, Svirče, Dol, Vrbanj, Bogomolje, Zastražišće and Gdinj.

In the year 1994. we had research and development of genealogy of Dol, Svirče and Jelsa.

During 2006 and 2007, surveys were conducted on 700 respondents from Bogomolje, Stari Grad, Sućuraj, Gdinj, Balote, Hvar, Poljica, Zastražišće, Svirče, Brusje, Vrbanj, Vrboska, Pitve, Dol, Vrisnik, Velo Grablje, Jelsa , Sv. Nedilja, Zaraće and Rudine.

From 2015 to 2018, he was involved in the project “Cohort of births on the Eastern Adriatic Islands (CRIBS)”. A longitudinal research approach was applied to a representative sample of 713 respondents in each of the two groups: pregnant women and their children in the target populations of the islands (Hvar, Brač and Vis) and the neighboring mainland area of ​​Split-Dalmatia County.

The project “Study of genetic, epigenetic and environmental risk factors for cardiometabolic traits in the island population” was conducted in 2017. This research is a continuation of the project from 2007 and 2008 on a population of 700 of the same respondents, and aims to compare changes in epigenetic characteristics over the past 10 years (2007/2008 vs. 2017).

Personal information, general health data (medical history, family history), genealogy data (family tree), WHO questionnaire for angina pectoris, WHO Claudication Questionnaire, WHO Questionnaire for Diabetes and Other Chronic Noncommunicable Diseases – Lifestyle Data (Smoking, Alcohol, Physical Activity, Reproductive Health of Female Respondents), Indicators of socio-economic status (education, working position, financial situation of the family), Questionnaire on eating habits (number of meals, way of processing food, consumption of fast food, fluid intake, diet, intake of vitamins and minerals, “food frequency questionnaire”), Blood pressure measurement (systolic and diastolic) , Anthropometry (body height, body weight, bicondylar upper arm width, abdominal circumference, hip circumference, upper arm circumference, skin folds, anthropometry of head and face, sitting height), Blood count, Biochemistry (creatinine, uric acid, cholesterol, triglycerides, HDL, LDL; calcium, HbA1c, insulin, fibrinogen), Genetics (GWAS analysis, Affymetrix Human SNP Array 5.0, refined data set for 344,512 SNPs, imputation using MACH software to 2.5 million SNPs).

Biological samples from the island of Hvar were collected in the field research of the Institute of Anthropology from the following projects: Integrated GWAS and EWAS of Cardiometabolic Traits in an Island Population (2016 – ZO17, National Institutes of Health (NIH), USA), Genetics of Metabolic Syndrome in an Island population (2006-2008, National Institutes of Health, (NIH), USA), Koster Health project (1994, University of Gothenburg, Sweden) and the First Croatian Health Project (1995-1997, World Bank) . In addition to the above, there are additional samples of collected blood from NIH projects that were originally collected in suitable tubes as a source for DNA isolation, and the rest was stored for storage as a backup.

Samples from the Integrated GWAS and EWAS of Cardiometabolic Traits project in an Island population (2016-2017, National Institutes of Health) consist of DNA, serum, plasma and whole blood samples. DNA samples were arranged according to the ordinal numbers in the labeled boxes. The volume is about 100 µl in each sample. The samples were stored in the Laboratory of Molecular Anthropology. A total of 543 samples were collected. We also collected 46 new DNA samples that were marked with special ordinal numbers and are not part of this follow-up study. A total of 554 serum and plasma samples were collected, as well as 46 samples of new subjects.

Samples from the Genetics of Metabolic Syndrome in an Island population project (2006-2008, National Institutes of Health) consist of DNA, serum and whole blood samples. DNA samples were stored in the Laboratory of Molecular Anthropology. A total of 1425 samples were collected. Serum samples were stored in the Laboratory of Molecular Anthropology and each sample contained approximately 1500 µl of sample. A total of 1412 serum samples were collected.

106 DNA samples (dilution 1: 100) were collected from the Koster Health Project (1994), and they are located in an average volume of about 300 µl and stored in the Laboratory for Molecular Anthropology.

From the project The First Croatian Health Project (1995-1997) there are 75 DNA samples of about 100 µl also in the Laboratory for Molecular Anthropology.

Here we combine published research data from different areas of anthropology:

Family Resemblance in Cardio-Respiratory Traits 1993 Coll. Antropol.

Family Correlations of Metacarpal Bones – Heritable and Environmental Components of Total Phenotypic Variation 1997 Coll. Antropol.

Complex Segregation Analysis of Body Height, Weight and BMI in Pedigree Data from Middle Dalmatia, Croatia 2003 Coll. Antropol.

Path Analysis of Familial Resemblance in Blood Pressure in Middle Dalmatia, Croatia 2003 Coll. Antropol.

Inbreeding and Susceptibility to Osteoporosis in Croatian Island Isolates 2004 Coll.Antropol.

Holistic Anthopological Research of Hvar Islanders 2004 Collegium Antropologium

Segregation Analysis of Systolic and Diastolic Blood Pressure in Middle Dalmatia Island Population 2005 Coll. Antropol.

3000 years of solitude: extreme differentiation in the island isolates of Dalmatia, Croatia 2006 Europen Journal of Human Genetics

Chronic Respiratory Symptoms in Croatian Adriatic Island Metapopulations 2006 Public Health

Metabolic Syndrome in an Island Population of the Eastern Adriatic Coast of Croatia 2008 Coll. Antropol.

Prevalence of metabolic syndrome and related metabolic traits in an island population of the Adriatic 2012 Annals of Human Biology

Nutritional Habits of Croatian Island Populations 2012 Coll.Antropol.

Smoking Habits According to Metabolic Traits in an ISland Population of the Eastern Adriatic Coast 2013 Coll.Antropol.

Modeling Metabolic Syndrome Through Structural Equations of Metabolic Traits, Comorbid Diseases, and GWAS Variants 2013 Obesity

Dietary Patterns in Adults from an Adriatic Island of Croatia and Their Associations with Metabolic Syndrome and Its Components 2013 Coll.Antropol.

Fish and Shellfish Intake and Diabetes in a Costal Population of the Adriatic 2015 Coll.Antropol.

PCA of Heritability Estimates for 50 Biometrics Traits in Six Isolated Populations From Eastern Adriatic, Croatia 2016 Anthropology and Public Health

Maternal Physical Activity in Pregnancy and Newborns’ Anthropometry 2018 Coll. Antropol.

Mediterranean Diet in Pregnancy and its Association with Newborns’ Body Size in Dalmatia, Croatia 2018 Coll.Antropol.

Impact of Pre-Pregnancy BMI on Blood Glucose Levels in Pregnancy and on the Anthropometry of Newborns 2018 Coll.Antropol.

The association between maternal anthropometry and blood pressure in pregnancy 2019 MEBM

Association of perfluoroalkyl substances exposure with cardiometabolic traits in an island population of the eastern Adriatic coast of Croatia 2019 Sci Total Environ.

Adherence to Mediterranean Diet and Maternal Lifestyle during Pregnancy 2020 Nutrients